In vitro clonal micropropagation of Astragalus gorodkovii Jurtz., Astragalus gorczakovsky L. Vassil.

Abstract. Microclonal propagation of rare plant species solves the problem biodiversity conservation, because received explants can be introduced into natural conditions to maintain stability communities. The purpose research was obtain in vitro cultures plants from Fabaceae family: Astragalus gorodkovii and gorczakovskii. Materials methods: seeds were selected population Komi Republic 2004. Seeds gorczakovskii collected 2019 Perm region Sverdlovsk region. After sterilization scarification, placed on a hormone-free Murashige-Skoog medium. resulting seedlings medium 4 variants concentration phytohormones BAP NAA, germinated at room temperature 16-hour light day. Then transplanted onto 9 concentrations hormones. results. 1.5–2 months considered as optimal period for division transplantation. Variants containing 0.5–1 mg/l smaller amount NAA turned out best reproduction A. gorczakovskii, active branching observed without verification. showed most growth. originality this is selection composition germination This biotechnological method tool preserving genetic diversity communities Ural flora.